Animal L-FABP assay kits
Intended animals：Rat, Dog, Cat, Monkey, Pig *1
This is an ELISA kit to measure Urinary L-FABP (L-type Fatty Acid Binding Protein) by sandwich method with colorimetric analysis.
Technology Award winning product at the 42nd Annual Meeting of the Japanese Society of Toxicity 2015
L-FABP is gathering attention as a biomarker of drug induced nephropathy and nephrotoxicity in animal. *2
What is urinary L-FABP?
L-FABP is a carrier protein of 14kDa excreted in the proximal tubule and plays a role in the intracellular transport of FFAs to mitochondria and/or peroxisomes for metabolism. Conventional markers are excreted in urine due to tissue damage in the glomerulus and renal tubule. But L-FABPs are excreted in urine due to ischemia or low capillary blood flow and oxidative stress on renal tubule before the progression of tissue damage. Thus, L-FABP is considered as useful biomarker for early diagnosis of renal disease accompanying tubular dysfunction.
Human L-FABP was approved by Japanese MHLW in August 2011 and has been actually used in clinical practice since then. L-FABP is explicitly stated in KDIGO (Kidney Disease Improving Global Outcomes) international guideline and the guide line for chronic kidney disease published by Japanese Society of Nephrology. Recently, the social cognition of L-FABP is leaping as a promising urinary biomarker for diagnosis of kidney disorder and the prognosis prediction as well as in research field. In contrast, L-FABP has not been nominated as the non-clinical kidney biomarker recommended by PSTC (The Predictive Safety Testing Consortium). L-FABP can be a bridge between clinical field and non-clinical field and that should be endorsed by future studies.
Characteristics: Technical development for High Sensitivity ELISA assay enables user to measure L-FABP in animal urine
- Excellent in Sensitivity, Accuracy, and reproducibility
- Measurement Sample：urine
- Measurement wavelength：450 nm (reference wavelength 610 nm or over)
- Test time：about 3 hours
- Measurement method：enzyme immunometric assay (Sandwich ELISA Method)
Example data: The Change of urinary L-FABP in each drug-induced nephropathy model
Test animal: 6 week old male SD rats
- Gentamicin (200mg/kg, s.c.)
- Cisplatin (4mg/kg, i.v)
- Amphotelicin B (50mg/kg, i.p.)
*1: For Research Use Only. Not for use in diagnostic procedures. This product is developed by CMIC HOLDINGS Co., Ltd.
*2: Time-related changes of urinary L-FABP and other urinary renal biomarker in drug-induced nephropathy Yoshiyuki Suzuki et.,al.